FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14952482
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC380410.25441261189948267No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC358220.23957226633009826No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA270790.1811003684873187No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC258890.1731418235447466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN247020.1652033421608533No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA206210.1379102145048561No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT205610.13750894333128105No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA194330.12996504526806987No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA189240.12656092814557476No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG178680.11949855549065365No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT173960.11634188892519651No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC170640.11412152176474782No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT167560.11206166307372917No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT163520.1093597705049904No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT162200.10847697392312527No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC159020.10635023670317743No Hit
CAAGAAAGTACCCCAAGTGTCAACTCCAACTCTTGTAGAGGTCTCAAGAA158030.10568813926677859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21200.053.8778651
ACCGTTG26350.043.8798683
GCGATTT52050.040.27415594
ACCCGGC56500.040.0135885
GACCGTT29500.038.719012
GTCTAAC29750.035.3916661
CACCCGG65400.034.5679634
TCACCCG73050.032.4924163
CCGTTGA36050.031.9423834
ACGTGCG32700.031.7604738
CGGCCCG73550.031.3080398
GTTGACT102400.027.9089571
CGTGCGC39300.027.263799
CGGACAC49100.027.13510512-13
CTCGATT47600.027.11416648-49
CGCAAAA58450.027.0205861
AACACGT38850.026.7369655
CCAGTAG45250.026.6947235
ATTCCGT49000.025.7644852-53
ACTACCG64850.025.7248067
Produced by FastQC (version 0.11.5)