FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13639295
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA540240.3960908536694895No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA289900.2125476426750796No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT288400.21144787908759216No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC287300.21064138579010133No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC267940.19644710375426297No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC254510.1866005537676251No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT214310.15712688962296073No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198650.14564535776959148No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG195660.14345316235186642No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT186740.13691323488494092No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG176850.12966212696477347No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA170530.12502845638282625No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG167530.1228289292078513No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA165730.1215092129028663No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC160010.11731544775591407No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT152310.11166999467347835No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC151030.11073152974548904No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA149820.10984438711824915No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT141250.1035610711550707No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT140040.10267392852783079No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG137980.101163586534348No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG136820.10031310269335769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21400.054.4712071
ACCGTTG26050.044.3841323
GCGATTT48850.040.02479694
GACCGTT29650.038.8392142
ACCCGGC55600.037.02585
CACCCGG57100.035.970174
GTTGACT99550.034.0898481
CCGTTGA34200.034.0818184
TCACCCG63550.033.133163
GTCTAAC37700.030.296611
CGCAAAA50450.029.720671
ACGTGCG40200.028.4071678
ACTACCG71950.028.0851927
CTCGATT43250.027.44899448-49
GACTATT123400.026.9278344
CGGACAC46300.026.08645212-13
ATTCCGT45350.025.86732752-53
CTACCGT78350.025.7315568
AACACGT45900.025.0875665
TTGACTA132400.024.5667212
Produced by FastQC (version 0.11.5)