FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004827494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13682556
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA540480.39501391406693315No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA291700.21319116106668956No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT291210.212833040844123No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC289450.2115467314732715No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC268290.19608178471917087No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC255580.18679258466035148No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT213420.15597962836768217No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210260.15367011836092614No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG195260.14270725440480567No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT185540.13560331856123958No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG180210.13170784756883144No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG169000.12351493390562406No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA168810.12337607096217988No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA166860.12195089864788422No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC157810.11533663739435819No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC157130.11483965422834738No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT151770.110922257508027No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA151500.11072492595681685No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT143240.10468804220497983No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT141260.10324094416277192No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG140540.10271472669287814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)