FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14213316
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA260900.18356026137742945No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC260620.18336326301336014No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN233250.16410667292558612No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG179340.12617745218638635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15650.033.93271
GTTGACT100700.028.9345381
GTAGGTC42250.027.2521882
GACTATT105000.026.0932984
TTGACTA103550.025.9601922
CCGTTGA22500.023.8106964
GCGATTT26450.023.4567894
ACATGCC126800.022.1637842
TACCGTA60700.021.7554159
TGACTAT126550.021.686823
GACCGTT24650.021.5438582
CTACCGT62550.021.4124978
ACTACCG60400.021.3187547
GCCTATC129400.020.8073376
ACCGTTG27250.020.5222783
ACCCGGC35500.020.518785
TCACATG94000.020.448312
CACATGC143800.020.066141
ATCACAT100300.019.8664041
TACTATA53050.019.7546444
Produced by FastQC (version 0.11.5)