FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14213381
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT152450.10725808306974954No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG143400.10089084363530394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT45900.032.811381
GTATCGA46800.031.0335254
CGTATCG52250.030.8550513
CCGTATC56600.028.8988972
TATCGAA53700.027.571145
ATCGAAG66700.022.3377656
TCGAAGG79700.019.2240837
CGCGTAT1851.634289E-417.8079321
TCAATCG37500.017.0448848
TTGCACG30900.016.730958
CCGTTAA20100.016.48466730-31
GTCGTCT38550.016.359411
GTCCGGT12800.015.4428171
CGAAGGC95050.015.4272618
GTCCGTT15200.015.1718911
GTCGCGT12650.014.5097961
CGCGTAA9200.014.3050273
GTCGATT11400.014.0365791
TCTACGG43900.013.703517
GTCCGCT19750.013.5829761
Produced by FastQC (version 0.11.5)