FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14992409
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA766480.511245390917497No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT574100.38292711998452017No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT535690.3573074880761324No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG312820.20865225861967882No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC304090.2028293118204019No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA280270.18694127141275294No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA263650.17585566135502306No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG249080.1661374099385896No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG230160.15351769018574665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN223620.1491554826179035No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC212720.1418851366714982No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT197370.13164662196715685No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT197230.13155324137701954No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT195480.13038598400030307No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG191740.12789138823520624No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC189310.12627056799210853No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG186020.12407612412388162No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG161880.10797464236734736No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT158580.10577352845696779No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG157220.10486640272420529No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA153870.10263193860306238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23300.054.4573671
CACCCGG117800.049.9479944
ACCCGGC121500.048.4652985
GCGATTT114950.047.31080294
TCACCCG126200.047.1073533
ACCGTTG30100.042.3116873
CCAGTAG70050.038.1733475
GACCGTT33400.037.8512952
CGGCCCG157550.037.7042738
GTAGCAT76950.035.2394458
CGGACAC105950.033.6226812-13
TAGCATA83500.033.0940449
CCCGGCC188350.031.6346666
CTCACCC187800.031.6069562
CTCGATT111050.031.35387448-49
CGGACAG113300.031.29669618-19
GACACGG114700.031.03746214-15
ATTCCGT112950.030.3047252-53
CCGGCCC201400.030.0083397
GTTGACT100000.029.5596641
Produced by FastQC (version 0.11.5)