FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14951466
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA567010.3792337152758131No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA460790.30819051456225094No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT252430.16883294253553463No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC242000.1618570379653741No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA227450.15212555076538983No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC190020.12709121633958836No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG170680.11415602991706633No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT161450.10798272222937871No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC155780.10419045195969412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT48500.034.9403761
GTATCGA47800.034.7112054
TATCGAA53000.031.2168945
TGTCGAG14150.028.2348445
CCCGTCG29800.025.7476841
GTCGAGG16350.025.2967416
AGTTTAC298750.023.53494394
CGGTTCG9700.023.2590713
CACGGAA60100.022.99123894
CGTATCG114850.021.8131873
CCGTATC114000.021.6459852
CGCGTAT2206.2176696E-821.3965681
ATCGAAG102600.020.7057386
CGCGTAA71100.018.6700861
GCGTAAC75250.018.6136972
TATCGCG3700.018.418586-87
CTTCGCG26150.018.1530386
TCGAAGG119250.018.0902187
TCGCGTA57550.017.8863492
GTCGGTA8000.017.652171
Produced by FastQC (version 0.11.5)