FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15014386
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA573970.38228003462812266No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA470870.313612557982724No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT256630.17092274036380842No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC252080.16789231341195038No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA231710.15432532505824748No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC191680.1276642281609118No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG172300.11475660742970109No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT165260.11006777100308997No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC156050.1039336540302081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT48400.033.059881
GTATCGA49750.032.1217584
TATCGAA53950.029.7953225
TGTCGAG14200.026.4798265
CGGTTCG9250.025.9144443
CACGGAA64800.024.87765594
CCCGTCG33850.024.3302821
AGTTTAC306450.022.23815394
CGTATCG119200.021.2927113
CGCGTAA74100.021.2127041
CCGTATC118000.021.0710952
GCGTAAC79300.020.44842
CTTCGCG28200.019.8331876
GTCGAGG19000.019.2945946
GTCGTAT7850.019.1843951
ACGTTCG15600.018.9806296
TCGACCG15500.018.95106330-31
ATCGAAG103550.018.926956
CGTAACT86400.018.8224353
ACGACTT21250.018.6032221
Produced by FastQC (version 0.11.5)