FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15014386
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA777800.5180365017923477No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT580180.38641606789648275No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT542900.361586547728292No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG317930.21175025072620351No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC306680.20425743683424682No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA282860.18839265222034388No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA268320.1787086065324283No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG252620.1682519684787643No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246560.1642158393956303No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG229600.15292000618606713No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC214130.1426165545497498No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT198990.1325328921209299No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT197870.13178694087124176No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT196510.13088114292519187No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG196060.1305814303695136No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG193070.12859000694400688No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC191900.1278107542992434No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG162340.10812296953068877No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG160990.10722383186365396No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT158470.10554544155185566No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA155240.1033941714299872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20250.055.710351
CACCCGG124000.051.211664
TCACCCG129800.049.467823
ACCCGGC127850.049.297745
GCGATTT123650.048.46643494
ACCGTTG28050.040.2184333
CCAGTAG68350.039.470475
CGGCCCG164400.038.7909668
GACCGTT29400.038.691692
GTAGCAT80100.033.970248
CGGACAC112150.033.71120512-13
TAGCATA83450.032.8318069
CTCACCC195100.032.814782
CCCGGCC196450.032.7969636
CGGACAG116250.032.6245118-19
CTCGATT117600.032.02341548-49
GACACGG121550.031.1821714-15
GTTGACT99000.031.0047071
ATTCCGT119450.030.99582752-53
CCGGCCC212300.030.5033447
Produced by FastQC (version 0.11.5)