FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14567257
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210500.1445021530134328No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT193410.13277036301343484No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC168780.11586258140430968No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA153450.10533898042713188No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA147360.1011583718197599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16250.037.595641
ACCCGGC57900.035.956235
GCGATTT53500.034.61430794
TCACCCG58350.034.3899463
CACCCGG67950.030.4308224
ACCGTTG23250.027.2869683
CGGCCCG76450.027.170078
CTCGATT46900.025.115448-49
GACCGTT24900.024.5358982
CATTCGG26950.024.4129184
ATTCCGT50150.023.15877352-53
CGGACAC54150.022.47804512-13
CCGTTGA28050.022.2827724
GCCTATC82850.022.0059646
TCGATTC48950.021.51804450-51
GCGGGTC47050.021.3752962
CTTAGCG14300.021.0324548
CCAGTAG58600.020.8508975
GTAGGTC29600.020.6399942
CGTTCTT56400.020.33132676-77
Produced by FastQC (version 0.11.5)