FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14549723
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT31300.030.5298161
GTATCGA29450.029.3665054
CCCGTCG20000.023.3011681
TATCGAA40850.023.0121755
CGTATCG54100.021.9807743
CCGTATC59100.020.359742
ATCGCGT6100.020.03248894
CGCGTAT2502.3931534E-718.8292271
GTCCGAT8350.018.6037271
CCCGGTT18900.016.936341
ATCGAAG68350.015.6095596
TATCGCG2609.094947E-1114.46122486-87
GTCCGCT20050.014.3214811
CGGTTCG5904.7293724E-1113.5430553
TCGACCG7700.013.42827930-31
CCGTCGG35850.013.1108432
CGAAAGG49150.013.1004288
CGTGCCG62900.013.00137368-69
TCGAAGG82450.012.8256757
ATCGCGC39750.012.76966466-67
Produced by FastQC (version 0.11.5)