FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14505278
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN224230.15458511033018466No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT192530.13273099626218815No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC168760.11634385773233716No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA154610.10658878788810527No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA147300.10154924297210988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16100.038.8306161
ACCCGGC58250.036.7936135
GCGATTT54800.036.62463494
TCACCCG62000.034.7983783
CACCCGG70750.030.2283174
CGGCCCG78750.027.630618
GTAGGTC30950.027.3375972
ACCGTTG24000.026.6357963
CTCGATT49550.025.52425848-49
GACCGTT25200.024.8084492
ATTCCGT50400.024.25338452-53
CCGTTGA28200.022.8349724
CGGACAC56500.022.45767812-13
CCAGTAG55600.022.3168375
GCCTATC79200.022.2516946
CGTTCTT57300.021.61166676-77
AGTACGC33750.021.03525448-49
TCGATTC51150.020.81934750-51
CTTAGCG14450.020.8147968
CGTGGGT59750.020.56918156-57
Produced by FastQC (version 0.11.5)