FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090739
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA245880.20336225932922708No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA199040.16462186471811194No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA195500.1616940039810635No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT191410.1583112496266771No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG172240.14245613936418608No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA170550.14105837534000196No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG148330.12268067319954554No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT147400.12191148944659214No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT147340.12186186468833707No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT142490.1178505300627199No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG138510.11455875443180105No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT136480.1128797834441716No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG135030.11168051845300772No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG131330.10862032502727914No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA129850.10739624765698773No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG121920.10083750877427756No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA121390.10039915674302456No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA121160.10020892850304683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG15900.036.6519475
ACGACTT45400.032.3699571
TGTTACG18250.032.1899033
GTTACGA19900.029.284724
TTACGAC19850.029.1217255
GTATCGA10850.027.7218844
CCCGTAT14600.026.1321751
TTCGCGC33100.025.6994067
GTCGAGG26050.024.1751736
TACGACT24550.023.5464866
CGCCCTT16950.022.509131
GTCGGTA6500.022.464241
GTTCGAC60100.021.27076328-29
GTCCGAT4700.021.045771
TCGACCG62050.020.79166430-31
TGTGTCG29200.020.7624863
TATCGAA15250.020.3397965
TTACGGA96800.020.1000678
AAGTTCG63650.020.01044826-27
AGTTACG98100.019.881616
Produced by FastQC (version 0.11.5)