FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090739
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT837790.6929187703084154No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG444610.36772772946302124No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206140.17049412777829379No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA188970.1562931761243047No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT122330.10117661128902046No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC121090.10015103295174926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG144750.058.3625453
CACCCGG149050.056.4218144
ACCCGGC167050.050.3682985
TAGCATA94500.047.195889
GCCAGTA96800.046.0364384
GTAGCAT98400.045.8506858
CCAGTAG98950.044.891775
CTCACCC204750.041.260142
CAGTAGC111600.039.840566
CCTCACC218850.039.3751681
GCGATTT198300.038.91576494
CGGCCCG217700.038.750368
TGCCAGT121050.036.894663
CGGACAC147900.032.39620612-13
GTCTAAC27650.031.6245481
GACACGG158400.030.3831314-15
CGGACAG158750.030.31765218-19
CCCGGCC292500.029.4533796
ACGGACA150850.028.88309518-19
CCCGGAC167000.028.8595710-11
Produced by FastQC (version 0.11.5)