FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12263855
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT838210.6834800313604491No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG449950.366891161058248No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN204630.16685618021413332No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA190540.15536713374383504No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT125120.10202338497968216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG139450.057.227323
CACCCGG141450.056.2507934
ACCCGGC161700.049.0886655
TAGCATA97100.044.386119
GCCAGTA99650.043.4623874
CCAGTAG100800.042.8253565
GTAGCAT103550.041.939088
CTCACCC196450.040.622972
CCTCACC207900.039.086731
CGGCCCG208300.038.4031878
GCGATTT195350.037.7230394
CAGTAGC116400.037.14866
TGCCAGT128250.033.844213
GTCTAAC28250.032.7935071
CGGACAC143100.031.66132212-13
GACACGG153100.029.82238814-15
CGGACAG153400.029.59731518-19
ACGTGCG32000.028.3468198
ACGGACA145700.028.29049518-19
CCCGGAC161250.028.2141710-11
Produced by FastQC (version 0.11.5)