FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12126390
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA244290.20145319423175403No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA201610.1662572290681728No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA201360.16605106713539644No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT190250.15688923084281473No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG170460.14056945224423756No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA168530.13897788212320403No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG150940.1244723285330589No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT150170.12383734978010769No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT146090.12047278703719738No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT143110.11801533679850311No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG142280.11733087918168557No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT138990.1146177881463486No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG135020.11134393665385989No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG133130.10978535244207056No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA128490.10595898696974121No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG124250.10246248058985402No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA123070.10148939626714959No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA122990.10142342444866115No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG122590.10109356535621895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG17750.033.6265033
ACGACTT43900.033.368911
GTTACGA18450.032.35074
TGTCGAG17400.031.0616785
TTACGAC19450.030.9290585
TACGACT22200.027.3094566
GTATCGA9450.026.8558124
CCCGTAT15200.025.7205771
CGCCCTT15800.024.4457261
GTCGAGG24600.024.2630256
TTCGCGC31450.024.0592217
GTCGTAT4900.023.070731
AGTTACG98400.021.9226936
TTACGGA96350.021.8037978
GTTCGAC64250.021.6884228-29
TCGACCG65000.021.40201630-31
GTTACGG98600.021.353917
TGTGTCG26750.020.9073753
TACGGAT103100.020.4218799
AAGTTCG68250.020.41730326-27
Produced by FastQC (version 0.11.5)