FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13187747
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG408620.30984822502281856No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA149800.11359028953163873No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA146100.11078465487698543No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC144830.10982163973876659No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT137150.10399805213127003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT22800.040.0797961
GTATCGA21100.037.6423844
GTTACGA13700.028.472814
TGTCGAG12200.028.1213325
TTACGAC14700.027.1753065
TGTTACG14300.026.9494973
CGTACGC38950.026.3040055
CCGTACG39900.025.7955064
TATCGAA32950.025.5311555
TACGCCA42050.024.4765937
CCGTATC61200.024.1158242
CGTATCG62100.023.9905663
GTACGCC42800.023.9378766
GTCGAGG15150.023.8864146
GAAATAA165650.023.57765294
TCCGTAC43400.023.4986483
ACGACTT27950.022.245941
TCGACGA122400.020.84944744-45
GACGAAA123450.020.71096846-47
TACGACT18650.020.6636896
Produced by FastQC (version 0.11.5)