FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004827540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13337208
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT553250.4148169541931115No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT464610.3483562676686155No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT389620.29213010699090847No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG299220.22434980394697301No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT295180.2213206842091688No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT291340.2184415208940282No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT247070.1852486667374461No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT242500.18182216247958344No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN227930.1708978370885421No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT181700.13623540998985695No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC164340.12321919250265874No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT162590.12190707380435245No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT158730.11901291484694547No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT157250.11790323731923502No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC152960.11468667205310136No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC136300.10219530204522566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)