FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13179637
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG413750.3139312562250387No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA153370.11636891061567174No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA146750.11134601051607113No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC144260.10945673238193131No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT137250.10413792124927264No Hit
CTCCAAAAAGGGTATGAAGTGATTTGTCACAATTTTCAGCTGACTCATCA132010.10016209095895434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24200.039.511081
GTATCGA22400.035.668174
TGTCGAG12450.029.4445655
CGTACGC33850.028.184955
CCGTACG36050.026.3345574
TCCGTAC37850.025.82723
GTTACGA12850.025.6020554
GTACGCC37800.025.2396976
TATCGAA33950.025.0564025
GTCGAGG14800.024.7692456
TACGCCA38900.024.5259767
CCGTATC61300.024.3066352
GAAATAA172600.024.09917694
CGTATCG61500.023.9193363
ACGACTT28250.022.8423291
TTACGAC14050.022.7463915
TGTTACG14500.022.3645943
TTCCGTA46450.021.755992
ATCGAAG70350.021.5783566
CCCAAAT239450.020.6347351
Produced by FastQC (version 0.11.5)