FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13179637
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT550280.4175228801825119No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT455130.3453281755787356No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT384480.2917227538209133No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT295580.22427021320845178No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG294160.22319279354962507No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT287130.2178588074921942No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN255420.19379896426585952No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT242050.18365452705563892No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT238740.18114307700583865No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT178190.135200992257981No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT162420.12323556407509555No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC157640.119608757054538No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT156980.11910798453705516No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT153770.11667240911111589No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC148940.11300766477862782No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC138000.10470698092823043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17500.051.578631
ACCCGGC105650.051.484185
TCACCCG111300.049.168013
CACCCGG113350.048.1537974
CGGCCCG127600.042.6584478
CCAGTAG67350.042.579665
ACCGTTG22200.042.1429443
GCGATTT125100.039.3699894
GACCGTT23250.039.2294162
CGCAAAA54000.038.305891
TAACCCC55900.036.3258489
TAGCATA82650.035.0333489
CTCACCC161400.034.1977582
GTAGCAT86250.033.5713468
CGGACAC99150.032.28407312-13
CTACCGT51400.031.5502748
CCCGGCC177750.031.3077076
TTAACCC64500.031.0453838
CGGACAG103250.030.7724218-19
CCGGCCC184650.030.6499167
Produced by FastQC (version 0.11.5)