FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12135147
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG150870.12432482276481693No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA134430.11077739725773407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT36650.043.949821
GTATCGA34200.043.562064
TATCGAA41850.036.1606035
CGTATCG70500.029.3318063
CCGTATC72900.028.3689582
GTTACGA12400.026.5308784
TGTCGAG12100.026.0234415
ATCGAAG79100.025.429786
TTACGAC13650.024.101315
GTCGAGG13150.023.9455226
ACGACTT28150.023.256341
CGTACGC42800.022.7301275
CCGTACG43250.022.3849644
TCGAAGG95650.021.1771557
GTACGCC46800.020.9882246
TCCGTAC47800.020.9424253
CGAAGGC72500.020.9382238
TACGCCA46750.020.9101437
TCGACCG33800.020.7879830-31
GTTCGAC34200.020.40742328-29
Produced by FastQC (version 0.11.5)