FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12284578
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG153070.12460338482933643No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA138330.11260460066271712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT36550.042.0061381
GTATCGA37150.038.711274
TATCGAA44500.032.9510575
CGTATCG72900.027.59253
CCGTATC73000.027.3644582
ACGACTT30800.024.0066451
TGTCGAG13550.023.5854645
ATCGAAG82450.022.7434866
CGTACGC43400.022.415865
CCGTACG43700.022.2619764
TCCCGTG51050.021.4503789
GTCGAGG16350.021.2710426
GTACGCC46550.020.7980386
TACGCCA47050.020.7767947
TTACGAC14600.020.6016465
TCGACCG33000.020.50841930-31
GTTCGAC32950.020.46822428-29
TCCGTAC48300.020.1417873
CGATCCG33550.019.89204882-83
GTTACGA15300.019.3519134
Produced by FastQC (version 0.11.5)