FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12284578
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT604370.49197457169468906No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC316790.2578761761291271No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC309990.2523407804484615No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG283030.2303945646321754No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG219040.1783048632195587No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT201080.1636849063923889No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT191210.15565044236765807No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA179850.1464030754658402No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT177070.14414007546697982No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166580.13560091360077653No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG164480.13389145316998272No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC146860.1195482661268462No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG143020.11642239562482326No Hit
CCCATATTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACAC141440.11513623015784505No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG138260.11254761864835731No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA124830.1016152121790427No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA123510.10054069419397232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT29000.058.3734741
ACCCGGC114550.052.9485745
ACCGTTG33550.050.7362333
GACCGTT33500.050.6728322
TCACCCG124500.049.5903933
GCGATTT122150.045.70772694
CACCCGG133500.045.610984
CGGCCCG137650.044.3175478
CCAGTAG64700.042.366635
CGCAAAA52850.036.8354841
TAGCATA79200.035.3671579
CTACCGT56800.034.917368
CAGTAGC78750.034.9143376
GTAGCAT80300.034.8239978
CGTTGAC49650.034.1859445
CGACCTA8950.033.6231084
CGGACAC104550.033.489712-13
GCCAGTA82050.033.2949564
CCGGCCC195800.032.163827
CGGACAG111350.031.21314818-19
Produced by FastQC (version 0.11.5)