FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10907381
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG131900.12092728767794944No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG119820.10985221841980214No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA115760.10612996832145132No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG113860.10438802862025266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT35700.041.427521
GTATCGA34600.040.6134574
TATCGAA39400.036.5006035
CGTATCG60500.029.8298243
CCGTATC61150.028.516232
CGTACGC33050.027.5870425
CCGTACG33450.027.257154
ATCGAAG67700.026.9350596
GTACGCC35950.025.361666
TACGCCA36650.025.0054977
TCCGTAC38050.024.0854533
GTTACGA11050.022.9671124
TTACGAC11350.022.7741265
TCCCGTG43650.022.2874779
TCGAAGG80950.021.9457197
TGTCGAG10350.021.3418665
TTCCGTA44100.021.316022
CTCGAGT29600.020.049631
CGAAGGC71650.020.0059198
CGAGTGA25450.019.9439353
Produced by FastQC (version 0.11.5)