FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10907381
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT431030.39517277337245305No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC329560.30214402522475375No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC287300.26339961902861925No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG210420.19291523785590692No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT198240.18174848756085443No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG181480.1663827457755441No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT177580.16280718533624158No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA177520.16275217671409847No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG172310.1579755946913379No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170960.1567379006931178No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC158120.14496605555449105No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT157150.14407674949651067No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG154850.14196808564769123No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT128660.1179568220822212No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC122250.11208006761659833No Hit
CCCATATTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACAC118910.10901792098396489No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT115680.10605662349192717No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG112730.10335203290322398No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT110590.10139005871345283No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA110030.10087664490678377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT27550.056.820211
ACCGTTG31100.050.3340643
GACCGTT31600.049.3891072
ACCCGGC83500.048.299555
GCGATTT84950.044.09358294
TCACCCG93200.043.6797523
CACCCGG100650.040.2579544
CGCAAAA48350.039.6683271
CGGCCCG104700.038.917678
ACTACCG56300.038.149627
CCAGTAG46900.038.084925
CTACCGT55900.037.9174548
CGTTGAC45700.034.147915
CGGACAC73550.032.68469212-13
GTAGCAT58900.030.8794888
TAGCATA59450.030.593819
GACACGG80600.030.14726314-15
CGGACAG80400.029.96153818-19
CTCGATT79650.029.95231648-49
ATTCCGT80800.029.49936752-53
Produced by FastQC (version 0.11.5)