FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11016700
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG131020.11892853576842431No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG119100.10810859876369512No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA117630.10677426089482332No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG116180.10545807728267086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT38300.045.7460751
GTATCGA36900.044.068274
TATCGAA44400.037.365265
CGTATCG62950.032.327233
CCGTATC62850.032.233062
ATCGAAG71300.027.684466
CGTACGC36800.024.7759445
CCGTACG37300.024.6958244
TGTCGAG10950.024.4645395
GTCGAGG11850.023.3996836
TCGAAGG84450.023.3179667
GTACGCC40700.022.6327866
CTCGAGT30800.022.6318781
ACGGCGA32950.022.25281394
TACGCCA41100.022.1838137
TCCGTAC41100.022.1838133
CGAGTGA25550.021.5214373
CGAAGGC77450.021.4811828
GACGAAA37950.021.05364246-47
TCGACGA38950.020.7544944-45
Produced by FastQC (version 0.11.5)