FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004827552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11016700
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT436160.39590803053545975No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC334790.30389318035346335No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC292930.2658963210398758No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG215690.19578458158976825No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT199300.18090716820826563No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA180810.16412355787123184No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT180630.16396016956075776No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG180290.16365154719652888No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG177690.16129149382301416No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166400.1510434159049443No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC159690.1449526627756043No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT158010.1434277052111794No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG157550.14301015730663447No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT128170.11634155418591774No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC124220.11275608848384726No Hit
CCCATATTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACAC119630.10858968656675774No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT117510.10666533535450724No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG113450.10298002124048036No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA111470.10118274982526528No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT111050.10080151043415904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)