FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10894740
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG130530.11981011019996807No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG119330.10952991994301839No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA115880.10636325419422583No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG112510.10327001837584007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT33250.040.2284281
GTATCGA31200.038.5625654
TATCGAA38200.032.8494725
CGTATCG55200.029.1184023
CCGTATC56000.028.9573942
CGTACGC38200.025.7136335
GTTACGA11150.025.711984
CCGTACG38950.025.339174
TGTCGAG9850.025.2883325
TTACGAC11450.025.0383035
GTACGCC40950.023.9868356
TACGCCA42100.023.4432477
TCCGTAC42850.023.2522833
TCCCGTG46750.022.9210139
GTCGAGG11400.022.262276
ATCGAAG66500.022.1209246
TTCCGTA50500.020.7559152
TGTTACG13650.020.6585183
ACGGCGA34800.020.39361694
TCGACGA37150.020.05169144-45
Produced by FastQC (version 0.11.5)