FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11933745
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA263540.22083595719533136No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA246240.20633925058730515No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA212800.17831787087791803No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG205070.1718404406998809No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT203470.17049970482861834No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT182610.15301986090703296No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA177590.14881330211094673No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG175070.14670164311370823No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA170820.1431403134556671No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA163020.1366042260832622No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT161360.13521321261682734No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG151670.12709338099649356No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG151260.12674981742948252No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT144310.12092599598868586No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA142580.11947632532788323No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA135520.11356032829593728No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG128740.10787896004146227No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG124990.10473661034319068No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA124730.10451874076411051No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT120110.10064736593583992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT29050.044.101871
GTATCGA28600.040.2600484
TGTCGAG13500.038.642345
TATCGAA34000.034.4187165
GTCGAGG15850.033.209556
CGCGTAT1351.3018507E-831.4009651
ACGACTT33850.028.2469521
GTTACGA17050.026.737554
CGCCCTT15450.026.5231461
TTACGAC18050.025.5166175
TGTTACG18600.024.509423
CGTACGC88350.023.1396585
CCGTACG89000.022.9178544
TCCGTAC89650.022.8041153
ATCGAAG105100.022.5820266
CCGTATC103450.022.535412
CACGGAA114050.022.33488594
GTACGCC92000.022.3237866
TACGCCA92900.022.0569277
TTCCGTA94850.022.051442
Produced by FastQC (version 0.11.5)