FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11933745
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT991820.8311054073972588No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG428580.35913286231606256No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC225230.1887337126777889No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA222410.1863706657046887No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA207560.17392696089953322No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN199850.16746628991988685No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG199670.16731545713436982No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC175770.1472882150573856No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG159590.13373002355924316No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC154070.12910448480338738No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG144880.12140363314282314No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT141580.11863836540834416No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT134030.11231176801582404No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA121220.10157750144652831No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA120730.10116690108595415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG170250.059.0062073
CACCCGG173050.057.9403724
ACCCGGC181250.055.2908175
CCAGTAG84900.051.0466275
CGACCGT16750.050.2370341
CTCACCC215750.046.8243062
CAGTAGC97300.044.6321956
GTAGCAT97400.044.585438
GCGATTT204800.044.4271194
TAGCATA99350.043.7576379
CGGCCCG232700.043.2818458
GCCAGTA101750.042.733594
ACCGTTG20050.042.6714363
GACCGTT20600.041.3044742
TGCCAGT118550.036.5999453
GTCTAAC19450.035.7707371
CGGACAC163350.035.64850212-13
CCCGGCC295100.034.5763936
GACACGG171150.034.07913214-15
CGCAAAA42600.033.8777471
Produced by FastQC (version 0.11.5)