FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11931351
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA212110.17777534161889963No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT166290.13937231416626666No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA162740.13639695957314474No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG155000.12990984843208453No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT149880.12561863279355373No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA143570.12033004477028629No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA139400.11683505078343602No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG137380.11514203211354691No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT121640.10194989653728233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT34400.042.993231
GTATCGA32800.040.2628754
TGTCGAG11850.033.7110945
TATCGAA40600.032.7591555
GTCGAGG13500.029.5908536
ACGACTT31850.025.4359441
GTTACGA14350.024.2355084
TTACGAC14350.024.2355085
CCGTATC92200.024.165322
CGTATCG93550.023.661893
ATCGAAG95300.023.4739596
CGCCCTT15500.023.3984931
CGTACGC65000.022.6309765
CCGTACG65150.022.578874
TCCGTAC65650.022.5500813
TGTTACG16650.021.7344573
GTACGCC67950.021.6484686
TACGCCA68600.021.4433447
GTTCGAC36750.021.35683628-29
TCGACCG36750.021.2290430-31
Produced by FastQC (version 0.11.5)