FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11931351
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT680470.570320997177939No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG323160.2708494620600802No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC256250.2147703139401397No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC243190.2038243615496686No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT224980.18856204967903467No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA188170.1577105559965506No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187430.1570903412362942No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT182790.15320142706387566No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG176020.14752730013558396No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA161590.1354331123105841No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC144580.12117655410523083No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA138590.11615616705937157No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT125090.1048414383249642No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG120460.10096090543308968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT25100.057.143311
TCACCCG125500.053.0212753
CACCCGG126950.052.15554
ACCCGGC127300.051.935825
ACCGTTG28500.050.821013
GACCGTT30150.047.416072
CGCAAAA52100.046.845611
CCAGTAG69350.046.3110125
GCGATTT146400.041.73135494
CGGCCCG160600.041.3503048
TAGCATA78350.041.2700659
GTAGCAT80750.040.0431258
CAGTAGC82850.038.8586246
CTCACCC177650.037.35092
GCCAGTA92100.034.9752734
CGGACAC115000.033.53211612-13
CTACCGT56450.032.8863148
CGTTGAC44950.032.2204135
TAACCCC75650.031.9951389
CGGACAG120300.031.76300418-19
Produced by FastQC (version 0.11.5)