FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11810081
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT679970.5757538834831022No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG317000.2684147551570561No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC251690.21311454172075533No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC238710.2021239312414538No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT219360.1857396236317092No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN217420.18409695919951777No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA186100.15757724269630327No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT184160.1559345782641118No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG175020.14819542727945728No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA159260.13485089560351027No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC144070.12198900244629991No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA140240.11874601029408689No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT123190.1043091914441569No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG121130.10256491890275775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23050.056.2916071
TCACCCG128250.053.557523
ACCCGGC128400.053.530215
CACCCGG129000.052.9910284
ACCGTTG27700.048.3718533
GACCGTT28450.046.270812
CCAGTAG70450.044.9107865
CGCAAAA53950.043.482511
GCGATTT150650.042.11470494
CGGCCCG166950.041.302948
GTAGCAT81450.039.1846548
TAGCATA81350.039.0591479
CTCACCC183650.037.657642
CAGTAGC85000.037.379686
GCCAGTA91600.034.4393274
CGGACAC118850.033.9323112-13
CTACCGT57000.033.4802368
CGTTGAC41650.032.1696555
CGGACAG126200.032.0102318-19
GACACGG127250.031.87632214-15
Produced by FastQC (version 0.11.5)