FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12965263
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT565540.4361963193496345No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC296640.22879597583172823No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG289480.22327352711626444No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC280380.2162547724639292No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT236740.18259560180152148No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG204120.15743606589391979No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA197990.15270804765009394No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT192780.14868961778870202No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN188930.1457201446665602No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC160060.12345295270909659No Hit
CCCATATTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACAC149750.11550093507551677No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT140100.10805796997716127No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG140060.1080271183083598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT29150.054.5089151
ACCCGGC113800.052.333345
TCACCCG124550.048.3115123
ACCGTTG32400.048.170093
CACCCGG127900.046.7491874
GACCGTT34400.045.643342
GCGATTT125550.043.98468494
CGGCCCG135350.043.7511338
CCAGTAG69800.040.6748165
CGCAAAA62200.040.6613041
TAGCATA84400.034.2459879
GTAGCAT85850.033.941298
CTACCGT64600.033.393138
CCCGGCC183600.033.2016266
CAGTAGC86300.033.166426
CCGGCCC185150.032.846897
CGGACAC107150.032.67691412-13
CTCACCC193550.031.258982
CGGACAG112550.031.04852718-19
CTCGATT113800.030.21677248-49
Produced by FastQC (version 0.11.5)