FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13078296
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA161900.12379288555634466No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG156350.11954921344493198No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA131070.10021947813384864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT37850.042.5562631
GTATCGA37400.040.211864
TATCGAA47150.032.693995
CGTATCG81850.026.470253
CGCGTAT1807.348717E-926.1656041
CCGTATC82550.025.851912
ACGACTT30950.025.261011
ATCGAAG84650.024.0401276
CGTACGC44600.023.6041815
CCGTACG45250.023.1612534
TGTCGAG12500.022.9348345
GTTACGA14200.022.1749294
TACGCCA47900.022.1742387
GTACGCC48250.022.0133886
TCCGTAC48700.021.7134743
TTACGAC14900.021.4485745
TCGAAGG96450.021.050257
GTTCGAC31800.020.39534628-29
GTCGAGG14850.020.2548646
TCGACCG31950.020.22612230-31
Produced by FastQC (version 0.11.5)