FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12929857
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT568170.43942481343761186No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC296210.22908992728999247No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG284860.22031179463160344No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC284270.2198554864141189No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT233110.18028815013190014No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN221050.17096090080501278No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG205010.15855550451950087No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA197940.15308753994727087No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT192110.14857859603551687No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC161260.12471909008738458No Hit
CCCATATTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACAC147070.11374449075500218No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG142970.11057353534536384No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT138100.10680705904172025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT27650.054.4041371
ACCCGGC112600.051.893735
ACCGTTG31050.047.843113
TCACCCG123150.047.7166023
CACCCGG129800.045.2719544
GACCGTT33450.044.270052
GCGATTT123800.043.8081194
CGGCCCG140350.041.8624048
CCAGTAG66300.041.762255
CGCAAAA58800.040.53291
TAGCATA83150.033.860469
GTAGCAT83600.033.3407258
CAGTAGC84100.033.1956866
CCCGGCC183000.032.642676
CCGGCCC187450.032.221517
CGGACAC107100.032.08123812-13
CTACCGT60950.031.7724048
GCCAGTA88800.031.2873254
CTCACCC192650.030.8686142
CGGACAG113750.030.12144318-19
Produced by FastQC (version 0.11.5)