FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13693974
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT557830.40735435893189226No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT349190.2549953724170938No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG312060.2278812563832822No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT309540.22604103089431893No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT243260.17764017954174588No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN242350.17697565367073137No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT237090.17313454808662554No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT198170.14471328775708206No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT187570.13697265673207792No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC177530.12964096470462116No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT144700.10566691597340552No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA138000.10077425296703499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19450.055.589911
TCACCCG114650.051.0483173
CACCCGG116650.050.0499844
ACCCGGC117850.049.219815
GACCGTT24400.043.5417942
ACCGTTG24450.043.4525953
CGGCCCG143550.040.4658438
CCAGTAG71000.039.7234765
GCGATTT136000.037.63298494
TAGCATA81850.034.9105459
GTAGCAT83350.034.113258
CTCACCC178100.033.0730972
CGGACAC101950.032.77633712-13
CGGACAG108850.030.72282218-19
CGCAAAA47050.029.9743211
GACACGG113800.029.7355714-15
CTACCGT48500.029.5550338
CCCGGCC206500.028.5640146
ACGGACA104850.028.19660818-19
CCGGCCC213250.028.143627
Produced by FastQC (version 0.11.5)