FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13854604
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT560850.4048112815061333No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT354530.2558932756215912No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG316770.22863879761557965No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT314760.22718801634460284No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT245650.1773056812017146No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT242560.1750753756657354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232640.1679153009353425No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT200260.1445440086198061No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT192010.1385893093732596No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC177500.12811625651660632No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT150700.1087725062369159No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA141290.10198054018721862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19650.051.21131
TCACCCG113450.049.1150133
ACCCGGC113500.049.0478675
CACCCGG118650.046.881524
CCAGTAG74100.043.2108885
GACCGTT23700.042.063182
ACCGTTG24350.041.3252263
CGGCCCG140350.039.4817168
TAGCATA84700.038.2324379
GTAGCAT87300.037.0398148
GCGATTT131950.036.79339294
CGCAAAA47650.033.6516271
CTACCGT52700.033.1762248
CGGACAC101000.032.55027412-13
CTCACCC180750.031.0627982
CGGACAG107500.030.69330818-19
GACACGG110700.029.90949814-15
CAGTAGC109100.029.6389626
GTCTAAC19050.028.8805471
CTCGATT115850.028.2635948-49
Produced by FastQC (version 0.11.5)