FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13704675
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT560430.40893344789278113No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT347730.25373093488171006No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT312290.22787114615997825No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG308300.2249597308947494No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN267870.1954588488964532No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT246870.18013561065840672No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT241020.17586699429209376No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT198660.14495783373191995No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT188980.13789455058219183No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC175250.12787607148655478No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT148130.10808720381913471No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA137130.10006074569444369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19250.052.5097581
TCACCCG116000.050.016863
ACCCGGC118100.049.086425
CACCCGG119100.048.5965654
ACCGTTG22300.044.6982273
CCAGTAG70700.044.555735
GACCGTT24700.040.1651652
CGGCCCG146650.039.330378
GCGATTT130350.038.8298294
TAGCATA83700.038.0213179
GTAGCAT85000.037.439958
CGGACAC103350.032.4741812-13
CTCACCC181950.032.378972
CTACCGT48700.031.2738678
CGGACAG109500.030.79687118-19
GACACGG112150.030.19699114-15
CAGTAGC107900.029.4472486
CGCAAAA45800.029.0504721
CCCGGAC118850.028.51643810-11
ACGGACA105950.028.3020918-19
Produced by FastQC (version 0.11.5)