FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12731338
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA301160.23655015678634878No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA168470.13232701857416715No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT163030.12805409769185297No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC157790.12393826948903565No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT127940.10049218707413156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT22450.021.4003121
CTTAACG37300.021.0417863
ACCCGTT23000.020.2320692
GTCCGTT13150.020.0585041
AACGGTT41450.018.4815336
GTATCGA18650.018.3958134
TTCCGAT34000.017.8314137
CCTCTTG203200.017.7165873
GAACTCT198850.017.702369
TAACGGT43450.017.630835
CGTACGC41400.017.3686495
CTCCGAT56600.017.3094461
AGTTACG29700.017.248256
TTAACGG44550.017.1955014
TTACGGA30550.017.0760238
GCCCTCT183600.016.9576241
GTTACGG31100.016.9251527
GTCGGTA11550.016.7200721
CTCTTGA213850.016.6364864
TGAACTC213200.016.5549438
Produced by FastQC (version 0.11.5)