FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12608836
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA297160.23567599737200165No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA166360.13193922103515343No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT159060.1261496303068737No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC155920.12365931319909308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT22800.025.6139721
CGCGTAT1852.7640817E-722.9118861
GTATCGA19450.021.2638114
CGTATCG38400.020.9287363
TTCCGAT33300.020.3233997
CTTAACG36050.019.4249113
CCGTATC42500.019.2436122
CTCCGTT35050.018.8117871
TGTCGAG11150.018.1247355
GAACTCT193750.017.9016369
TCGACGA27400.017.75298144-45
AACGGTT40950.017.3301036
GCCCTCT181000.017.2774471
CCTCTTG205000.017.2631173
TAACGGT41300.017.1832355
CTCTTGA211600.016.9023484
GACGAAA29500.016.64872446-47
CGTACGC40450.016.6148175
TTAACGG42450.016.6070184
CCCGTCT35500.016.583331
Produced by FastQC (version 0.11.5)