FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11569975
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA147370.12737279034742943No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT144140.12458108163587217No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG144030.12448600796458073No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT132560.11457241696719311No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA129150.11162513315715894No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG122490.10586885451351451No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG119200.10302528743579827No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA118690.1025844913234471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG14200.032.104063
TGTCGAG13900.030.7682745
GTTACGA15250.030.2017964
TTACGAC15500.030.017885
CCCGTAT18250.028.9056281
GTATCGA15350.028.1679974
GTCGAGG15850.027.5759336
ACGACTT32400.024.4225791
CGTACGC47350.022.9281255
CCGTACG47600.022.8077054
CGTATCG60450.021.7689883
CCGTATC59450.021.267132
TACGCCA51400.021.2129657
TATCGAA21800.020.9118185
GTACGCC52000.020.7874416
GTTACGG61400.020.5901957
CGCCTCA40950.020.314024
CGTGCGT30450.020.29629986-87
TCCGTAC55000.020.1662833
TTACGGA62550.020.1365018
Produced by FastQC (version 0.11.5)