FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11569975
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT635390.5491714545623477No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG351260.30359616161659814No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG180380.15590353479588331No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163860.14162519798011663No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA138740.11991382868156586No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT137190.11857415422245944No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT121720.10520333881447452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG113600.052.677753
CACCCGG114100.052.1544
ACCCGGC117700.050.477185
CGACCGT15950.048.9245031
CCAGTAG75550.045.4786345
TAGCATA86750.040.0896459
CTCACCC150700.040.08382
GCGATTT135150.039.78121694
CGGCCCG150750.039.6865128
GTAGCAT91050.038.1448868
ACCGTTG20450.037.2390753
CAGTAGC93700.037.0173726
GACCGTT20600.036.7398762
GCCAGTA98000.035.2533844
GTCTAAC22150.033.321
CGGACAC110600.031.12614412-13
CGGACAG113600.029.89322318-19
GACACGG116050.029.50277514-15
ACGTGCG24400.029.4695478
CTCGATT118300.028.6526248-49
Produced by FastQC (version 0.11.5)