FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11757643
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA151260.12864823332363468No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT147900.12579051770835362No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG147670.12559490027040282No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT133550.11358569060142411No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA131070.11147642431395477No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG124180.10561640628142903No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA122530.10421306379178208No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG120760.10270766003016081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA15850.029.6513614
CCCGTAT20150.029.2168431
TGTTACG16150.028.8095553
TTACGAC16700.028.423585
TGTCGAG15300.027.9527055
GTATCGA15400.026.855664
GTCGAGG17900.025.4678676
CGTACGC51400.023.8644435
CCGTACG52100.023.5438064
TACGCCA53950.022.6493537
GTACGCC55800.021.9826566
ACGACTT34100.021.684211
TCGACGA46600.021.28075644-45
TCCGTAC58650.020.994583
CGTATCG60600.020.784333
GACGAAA48050.020.58957346-47
TTACGGA63700.020.5106438
TCCGACG10600.020.398052
GTTACGG63950.020.2099887
TACGACT23700.020.028436
Produced by FastQC (version 0.11.5)