FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11757643
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT649270.5522110171230747No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG356870.30352171774564No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG181100.15402746962124977No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN168460.14327701563995435No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA141170.12006658137179364No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT140640.11961581075390706No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT124570.10594810541534558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGG120450.053.598064
TCACCCG124700.051.9605453
ACCCGGC128500.050.2377975
CGACCGT16950.049.657351
CCAGTAG74450.045.7232445
GCGATTT142050.041.05920494
CGGCCCG162100.040.127868
CTCACCC164300.039.8383942
TAGCATA87000.039.7606359
ACCGTTG20950.039.502043
GACCGTT21300.039.2952962
GTAGCAT89200.038.5163768
CAGTAGC90350.037.9224136
GCCAGTA100750.034.1626364
CGGACAC115850.032.86108412-13
CGGACAG121500.031.3724618-19
GACACGG125750.030.51603914-15
CTCGATT126050.030.2269148-49
GTCTAAC20000.029.8589061
CCCGGAC130500.029.5322310-11
Produced by FastQC (version 0.11.5)