FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13521061
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT352960.2610446029346366No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN285870.21142571577777808No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG198660.14692633958237447No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG153150.11326773838236512No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC142040.10505092758623011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21850.048.6248171
TCACCCG89300.042.0621533
ACCCGGC93100.040.3399435
CACCCGG94250.039.7493174
GACCGTT28500.037.443982
GCGATTT89050.037.41889694
ACCGTTG29500.036.6523633
GTCTAAC31400.032.937761
CCAGTAG63950.031.6792535
CGGCCCG121500.030.7514978
ACGTGCG33450.030.0672078
GCCAGTA70500.028.9369874
CTCGATT76050.028.61975548-49
TAGCATA73550.028.3710699
CAGTAGC73050.027.8579756
CGGACAC80400.027.82489812-13
AACACGT38450.026.650075
CTACCGT34000.026.5398048
CTCACCC143200.026.5258542
TAACACG38650.026.3914324
Produced by FastQC (version 0.11.5)