FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13457286
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT357950.26598973968450995No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN294510.218847990597807No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG194400.14445706214462561No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG149130.11081729258039102No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC140220.10419634389876235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22050.047.331541
TCACCCG81950.042.797693
ACCCGGC85450.041.043195
CACCCGG88600.039.638224
ACCGTTG28300.037.2127763
GACCGTT28050.036.706672
GCGATTT87150.036.4001794
CCAGTAG63550.032.0321925
GTCTAAC29650.032.0282251
CGGCCCG116850.030.3310438
ACGTGCG31950.029.7183888
GCCAGTA70150.029.0193374
TAGCATA71750.028.4976379
CAGTAGC72950.028.2205856
CTCGATT72700.027.6140748-49
GTAGCAT78200.026.3276488
AACACGT35900.026.1908685
CGGACAC78700.025.98057612-13
TAACACG35650.025.8478134
CTCACCC137750.025.5978452
Produced by FastQC (version 0.11.5)