FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11049512
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT21250.028.372091
GTATCGA19050.024.6703534
CGTATCG34350.021.7540823
GTTACGA9100.021.6909334
TTACGAC9650.020.9416795
CCGTATC36550.020.7043652
TGTTACG9600.019.5820923
CGTACGC27750.018.9681665
CGACGCA7700.018.920884
TCGACGA19100.018.33221244-45
CCGTACG28900.018.2133794
TCCGACG9550.018.2104972
CTTAACG22800.017.7269463
CCCGATT8250.017.698971
GTTACGG27400.017.3237197
CCCGTCG25600.017.2953011
TATCGAA29500.017.205695
GTACGCC31500.017.1576426
TACGTGC12850.016.8238379
TCGACCG20350.016.62857830-31
Produced by FastQC (version 0.11.5)