FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11049512
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT274930.24881641831784065No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG154890.14017813637380547No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151370.1369924753237971No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC134570.1217881839487572No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC122180.11057501905966526No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA121630.11007725952060146No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG120350.10891883732059841No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT119060.10775136494715785No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC111270.10070127983932685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21100.052.593871
ACCCGGC68650.044.1074035
GACCGTT26200.042.715082
ACCGTTG26650.042.1698763
TCACCCG73650.040.9886783
CACCCGG74150.040.8372654
GCGATTT69200.040.40918794
CGGCCCG96900.031.0422368
CCGTTGA36800.030.9207124
CTACCGT37850.030.9193448
CCAGTAG50450.030.662035
CGTTGAC38800.029.689345
CTCGATT59700.028.82079348-49
ACTACCG42000.028.4237427
CGGACAC63550.027.80745512-13
CTCACCC114550.026.5181352
ATTCCGT65450.026.39796852-53
TAGCATA60550.026.0033579
GCCAGTA61450.025.633324
CGGACAG69050.025.52515218-19
Produced by FastQC (version 0.11.5)