FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13549807
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCT163470.1206437848155328No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT141400.10435572993770317No Hit
GGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTT140200.10347010846722762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT46500.030.0790751
CGTCATC62050.022.844292
GTAGGTC26900.022.2333242
CGACCGT6200.022.0276051
TACTCTA70900.020.08069
ACTACCG47050.019.1751847
CGGTAAT10750.017.9612451
CTACCGT55900.017.7361358
CAACCCG25750.017.3360753
CGCCGTA8900.016.63020946-47
TCATCTA91500.016.330914
TACCGTA58950.016.260239
GTAATCG10700.016.2488383
TCGCATA10200.016.1237417
AACCCGT28750.015.5270924
CCGTATG45850.015.4754054
GTTGACT56950.015.3808461
GGTCTAC42450.015.0544745
CGCAAGG81100.015.00669194
AGTAGGT42450.014.5329781
Produced by FastQC (version 0.11.5)